(1)英文名稱:MV-4-11細(xì)胞,別稱:MV4-11細(xì)胞;MV411細(xì)胞;MV-411細(xì)胞
(2)MV-4-11細(xì)胞中文名稱:人髓性單核細(xì)胞白血病細(xì)胞
(3)MV-4-11細(xì)胞描述:MV-4-11細(xì)胞系由Rovera課題組建立,來源于一名患有人雙表型髓性單核細(xì)胞白血?。╞iphenotypic B myelomonocytic leukemia)的10歲男孩的外周血。Interleukin (IL)-3可以獨(dú)立地支持該細(xì)胞長(zhǎng)期生長(zhǎng),使用10%FBS培養(yǎng)時(shí)則不需要額外添加IL-3。但是,在IL-3和生長(zhǎng)因子Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF)均處于低濃度的情況下,IL-3會(huì)抑制MV-4-11細(xì)胞的增殖。生長(zhǎng)因子Granulocyte Colony Stimulating Factor(G-CSF)會(huì)協(xié)同GM-CSF促進(jìn)MV-4-11細(xì)胞的增殖,而單獨(dú)的G-CSF會(huì)短暫刺激該細(xì)胞系。據(jù)文獻(xiàn)[PubMed: 3500218]報(bào)道,間接免疫熒光法檢測(cè)髓性單核細(xì)胞抗原CD15,超過96%的該細(xì)胞為陽(yáng)性,40~96%為單核細(xì)胞抗原CD4陽(yáng)性,4-11%為單核細(xì)胞抗原CD10陽(yáng)性。每個(gè)批次均通過本庫(kù)支原體檢測(cè),結(jié)果為陰性。經(jīng)本庫(kù)STR檢測(cè)無誤。STR鑒定結(jié)果為:Amelogenin: X,Y;CSF1PO: 10,12;D13S317: 13;D16S539: 11,12;D5S818: 11,12;D7S820: 8,9;THO1: 8,9.3;TPOX: 8,11;vWA: 14,15
(4)規(guī)格:T25方瓶或1ml凍存管
(5)細(xì)胞數(shù)量:1×10^6
(6)組織來源:人白血病
(7)生長(zhǎng)方式:懸浮生長(zhǎng)
(8)細(xì)胞形態(tài):淋巴細(xì)胞樣
(9)MV-4-11細(xì)胞完全培養(yǎng)基:IMDM+10%FBS+1%雙抗
(10)培養(yǎng)條件:37℃,5%CO2
(11)運(yùn)輸方式:常溫運(yùn)輸(T25方瓶)或干冰運(yùn)輸(凍存管)
(12)支原體檢測(cè):陰性
(13)STR profiling
